Leluo Guan

Leluo Guan

Canadian Research Chair (Tier 1) in Functional Genomics & Animal Microbiome


311A Food, Nutrition and Health building, 2205 East Mall, Vancouver

1985-1989 Wuhan University, PR China, BSc Biological Sciences

1990-1993 Kyoto University, Japan, MSc Pharmaceutical Science

1993-1996 Kyoto University, Japan, PhD Pharmaceutical Science

My research program are in the field of host-microbiome interaction and functional genomics in food producing animals, mainly cattle. My research focuses on 1) Elucidation of the molecular mechanisms of host-microbiome interactions by characterization of bovine gut microbiome and its functions using metagenomics/metatranscriptomics/metabolomics approaches, and host gene expression using a functional genomics approach; 2) Association between gut microbial ecology and feed efficiency, methane emission and gut immunity development in beef and dairy cattle; 3) Understanding of the bovine transcriptome, proteome, and non-coding RNAs and their roles in economically important traits. My core competence relates to microbial genomics, microbiome metagenomics and metatranscriptomics, microbial ecology, transcriptomics, proteomics and non-coding RNAs, cattle physiology and metabolism.

Guo W, Bi SS, Wang WW, Zhou M, Neves ALA, Degen AA, Guan LL, Long RJ. 2023. J Maternal rumen and milk microbiota shape the establishment of early-life rumen microbiota in grazing yak calves. Dairy Sci. Mar;106(3):2054-2070. doi: 10.3168/jds.2022-22655.

Zang X, Sun H-Z, Xue M-Y, Zhang Z, Plastow P, Yang T, Guan LL, Liu JX. 2022. Heritable and non-heritable rumen bacteria are associated with different characters of lactation performance of dairy cows. mSystems. Oct 26;7(5):e0042222. doi: 10.1128/msystems.00422-22.

Ma T, Zaheer R, McAllister TA, Guo W, Li F, Tu Y, Diao Q, Guan LL. 2022. Expressions of resistome is linked to the key functions and stability of active rumen microbiome. Animal Microbiome. Jun 4;4(1):38. doi: 10.1186/s42523-022-00189-6.

Pan Z, Chen Y, Zhou M, McAllister TA, Guan LL. 2021. Microbial interaction-driven community differences as revealed by network analysis. Comput Struct Biotechnol J. 19: 6000-6008.

Neves ALA, Yu J, Suzuki Y, Baez-Magana M, Arutyunova E, O’Hara E, McAllister T, Ominski KH, Joanne Lemieux M, Guan LL. 2021. Accelerated discovery of novel glycoside hydrolases using targeted functional profiling and selective pressure on the rumen microbiome. Microbiome. 9(1):229.

Malmuthuge N, Guan LL. 2021 Noncoding RNAs: Regulatory Molecules of Host–Microbiome Crosstalk. Trends in Microbiology. 29 (8):713-724

Xue M, Sun HZ, Wu X, Liu J, Guan LL. 2020. 2020. Multi-omics reveals that the rumen microbiome and its metabolome together with the host metabolome contribute to individualized dairy cow performance. Microbiome.8:64.

Ma T, Villot C, Renaud D, Skidmore A, Chevaux E, Steele M, Guan LL. 2020. Linking perturbations to temporal changes in diversity, stability, and compositions of neonatal calf gut microbiota: prediction of diarrhea. ISME J 14: 2223–2235.

O'Hara E, Neves ALA, Song Y, Guan LL. 2020. The Role of the Gut Microbiome in Cattle Production and Health: Driver or Passenger? Annu Rev Anim Biosci. Feb 15;8:199-220.

Sun HZ, Zhou M, Wang O, Chen Y, Liu JX, Guan LL. 2019. Multi-omics reveals functional genomic and metabolic mechanisms of milk production and quality in dairy cows. Bioinformatics. 2019 Dec 24. pii: btz951.

Malmuthuge N, Liang G, Guan LL. 2019. Regulation of rumen development in neonatal ruminants through microbial metagenomes and host transcriptomes. Genome Biology. 2019 Aug 23;20(1):172.

Li F, Li C, Chen Y, Liu J, Zhang C, Irving B, Fitzsimmons C, Plastow P, Guan LL. 2019. Host genetics influence the rumen microbiota and heritable rumen microbial features associate with feed efficiency in cattle. Microbiome. Jun 13;7(1):92.

Malmuthuge N, Liang G, Griebel PJ, Guan LL. 2019. Taxonomic and functional composition of the small intestinal microbiome in neonatal calves provide a framework for understanding early life gut health. Appl Environ Microbiol. Mar 6;85(6). pii: e02534-18.

Li F, Hitch TCA, Chen Y, Creevey CJ, Guan LL. 2019. Comparative metagenomic and metatranscriptomic analyses reveal the breed effect on the rumen microbiome and its associations with feed efficiency in beef cattle. Microbiome. 7:6.

Zhou M, Peng YJ, Chen Y, Klinger CM, Oba M, Liu JX, Guan LL. 2018. Assessment of microbiome changes after rumen transfaunation: implications on improving feed efficiency in beef cattle. Microbiome. Mar 27;6(1):62.

Li F and Guan LL. 2017. Metatranscriptomic profiling reveals linkages between the active rumen microbiome and feed efficiency in beef cattle. Appl Environ Microbiol. Apr 17;83(9). pii: e00061-17.

Liang G, Malmuthuge N, Bao H, Stothard P, Griebel PJ, Guan L L. 2016. Transcriptome analysis reveals regional and temporal differences in mucosal immune system development in the small intestine of neonatal calves. BMC Genomics. 17(1):602. doi: 10.1186/s12864-016-2957-y.

Kong RSG, Liang G, Chen Y, Stothard P, Guan LL. 2016. Transcriptome profiling of the rumen epithelium of beef cattle differing in residual feed intake. BMC Genomics. 17:592. doi: 10.1186/s12864-016-2935-4.

Malmuthuge N, Chen Y, Liang G, Goonewardene LA, Guan LL. 2015. Heat-treated colostrum feeding promotes beneficial bacteria colonization in the small intestine of neonatal calves. J Dairy Sci. 98(11):8044-53. doi: 10.3168/jds.2015-9607.

Zhou M, Grebiel P, Guan LL. 2014. Methanogen Prevalence throughout the Gastrointestinal Tract of Pre-Weaned Dairy Calves. Gut Microbes. 5: 628-638.

Zhou M, Hunerberg M, Beauchemin KA, McAllister TA, Okine E, Guan LL. 2013. Individuality of ruminal methanogen/protozoa adaptation to dried distillers‟ grain with soluble supplement in beef cattle. ACTA Agriculturae Scandinavica, Section A – Animal Science. 62:no. 453: 273-288.

Hernandez-Sanabria E, Guan LL, Goonewardene LA, Li M, Fujibi D, Stothard P, Moore SS, Leon-Quintero MC. 2010. Association Between Microbial Diversity and Microbial Fermentation Parameters in the Bovine Rumen and Host’s Feed Efficiency Traits. Appl Environ Microbiol 76: 6338 – 6350.

Zhou M, Hernandez-Sanabria E, Guan LL. 2010. Characterization of Rumen Methanogenic Community Variation under Different Diets and Host Feed Efficiencies Using PCR-DGGE Analysis. Appl Environ Microbiol. 76(12):3776-86.

Zhou M, Hernandez-Sanabria E, Guan LL. 2009. Assessment of Microbial Ecology of Ruminal Methane Producers and Cattle’s High Feed Efficiency and Low Methane Production Activities. Appl Environ Microbiol 75: 6524-6533.

Guan LL, Nkrumah D, Basarab JA, Moore SS. 2008. Linkage of microbial ecology to phenotype: correlation of rumen microbial ecology to cattle’s feed efficiency. FEMS Microbiology Letters 288: 85-9.

Dr. Guan is currently seeking graduate students to supervise. Please send her an email if you are interested.